مجله زیست شناسی ایران
پتانسیل درمانی سلولهای بنیادی
نویسنده
ارومیه، آموزش و پرورش استان آذربایجان غربی
چکیده
در سالهای اخیر سلول های بنیادی نه فقط در بین محافل علمی و پزشکی بلکه در میان سیاستمداران، گروه های مذهبی و دانشمندان علم اخلاق نیز مورد توجه و علاقه شدید قرار گرفته است. در اینجا ما انواع مختلف سلولهای بنیادی را که توصیف شده اند و همچنین منشا آنها را در بافت های بالغ و جنینی و قدرت تمایز آنها در بدن موجود زنده و محیط کشت به اختصار بیان می کنیم. در اینجا تعدادی از کاربردهای درمانی اخیر سلولهای بنیادی را با شناسایی مشکلات و مسایل رویارو به هنگام انتقال از اصول مستند و اثبات شده در آزمایشگاه به کاربردهای عملی درمانی گسترده، بررسی می کنیم. علی رغم اینکه تعدادی از عوامل ژنتیکی و اپی ژنتیکی تعیین کننده خواص سلول های بنیادی شناسایی شده اند، با این وجود مطالب زیادی برای دانستن اینکه این عوامل چگونه با یکدیگر تعامل و واکنش دارند وجود دارد. آگاهی فزاینده ای نسبت به اهمیت عوامل محیطی در کنترل و تنظیم رفتار سلول های بنیادی به وجود آمده است و اینها از طریق ترسیم سلول های بنیادی در بدن موجود زنده و خلق دوباره آنها در محیط زندگی مصنوعی در آزمایشگاه در حال کشف شدن هستند. درمان های جدید که بر اساس پیوند سلول های بنیادی یا سلول های بنیادی درونی (درونزاد) انجام می گیرد در مراحل اولیه اند که زمینه های نوظهوری هستند. همچنین اکتشاف داروهای جدید بر اساس سلول های پرتوان خاص یک بیمار و سلول های بنیادی سرطان صورت می گیرد. چیزی که مطالعه سلول های بنیادی را اینقدر هیجان انگیز می کند قدرت فوق العاده بالقوه آنها برای سلامتی انسان و ایجاد فرصت هایی برای مطالعه و تحقیقات بینابینی است.
کلیدواژه‌ها
Aasen, T. et al. 2008 Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat. Biotechnol. 26, 1276–1284. (doi:10.1038/nbt.1503)
Anderson, D. J., Gage, F. H. & Weissman, I. L. 2001 Can stem cells cross lineage boundaries? Nat. Med. 7, 393–395. (doi:10.1038/86439)
Andrews, P., Matin, M., Bahrami, A., Damjanov, I., Gokhale, P. & Draper, J. 2005 Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin. Biochem. Soc. Trans. 33, 1526–1530. (doi:10.1042/BST20051526)
Aoi, T., Yae, K., Nakagawa, M., Ichisaka, T., Okita, K., Takahashi, K., Chiba, T. & Yamanaka, S. 2008 Generation of pluripotent stem cells from adult mouse liver and stomach cells. Science 321, 699–702. (doi:10.1126/science.1154884)
Arias, A. M. 2008 Drosophila melanogaster and the development of biology in the 20th century. Methods Mol. Biol. 420, 1–25. (doi:10.1007/978-1-59745-583-1_1)
Austin, E. B., Guttridge, M., Pamphilon, D. & Watt, S. M. 2008 The role of blood services and regulatory bodies in stem cell transplantation. Vox Sang. 94, 6–17.
Barabe´, F., Kennedy, J. A., Hope, K. J. & Dick, J. E. 2007 Modeling the initiation and progression of human acute leukemia in mice. Science 316, 600–604. (doi:10.1126/science.1139851)
Brockes, J. P. & Kumar, A. 2005 Appendage regeneration in adult vertebrates and implications for regenerative medicine. Science 310, 1919–1922. (doi:10.1126/science.1115200)
Brons, I. G. et al. 2007 Derivation of pluripotent epiblast stem cells from mammalian embryos. Nature 448, 191–195. (doi:10.1038/nature05950)
Chambers, I. et al. 2007 Nanog safeguards pluripotency and mediates germline development. Nature 450, 1230–1234. (doi:10.1038/nature06403)
Chang, H. H., Hemberg, M., Barahona, M., Ingber, D. E. & Huang, S. 2008 Transcriptome-wide noise controls lineage choice in mammalian progenitor cells. Nature 453, 544–457. (doi:10.1038/nature06965)
Chen, C. S. 2008 Mechanotransduction—a field pulling together? J. Cell Sci. 121, 3285–3292. (doi:10.1242/jcs. 023507)
Clarke, M. F., Dick, J. E., Dirks, P. B., Eaves, C. J., Jamieson, C. H., Jones, D. L., Visvader, J., Weissman, I. L. & Wahl, G. M. 2006 Cancer stem cells–perspectives on current status and future directions: AACR Workshop on cancer stem cells. Cancer Res. 66, 9339–9344. (doi:10.1158/0008-5472.CAN-06-3126)
Clayton, E., Doupe´, D. P., Klein, A. M., Winton, D. J., Simons, B. D. & Jones, P. H. 2007 A single type of progenitor cell maintains normal epidermis. Nature 446, 185–189. (doi:10.1038/nature05574)
Conti, L. et al. 2005 Niche-independent symmetrical selfrenewal of a mammalian tissue stem cell. PLoS Biol. 3, e283. (doi:10.1371/journal.pbio.0030283)
De Luca, M., Pellegrini, G. & Green, H. 2006 Regeneration of squamous epithelia from stem cells of cultured grafts. Review. Regen. Med. 1, 45–57. (doi:10.2217/17460751.1.1.45)
Dick, J. E. 2008 Stem cell concepts renew cancer research. Blood 112, 4793–4807. (doi:10.1182/blood-2008-08-077941)
Dimos, J. T. et al. 2008 Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science 321, 1218–1221. (doi:10.1126/science.1158799)
Dor, Y., Brown, J., Martinez, O. I. & Melton, D. A. 2004 Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature 429, 41–46. (doi:10.1038/nature02520)
Dunnett, S. B., Bjo¨rklund, A. & Lindvall, O. 2001 Cell therapy in Parkinson’s disease—stop or go? Review. Nat. Rev. Neurosci. 2, 365–369. (doi:10.1038/35072572)
Evans, M. & Kaufman, M. 1981 Establishment in culture of pluripotential cells from mouse embryos. Nature 292, 154–156. (doi:10.1038/292154a0)
Gaspar, H. B. & Thrasher, A. J. 2005 Gene therapy for severe combined immunodeficiencies. Expert Opin. Biol. Ther. 5, 1175–1182. (doi:10.1517/14712598.5.9.1175)
Green, H. 2008 The birth of therapy with cultured cells. Bioessays 30, 897–903. (doi:10.1002/bies.20797)
Gurdon, J. B. & Melton, D. A. 2008 Nuclear reprogramming in cells. Review. Science 322, 1811–1815. (doi:10.1126/science.1160810)
Gurdon, J. B., Elsdale, T. R. & Fischberg, M. 1958 Sexually mature individuals of Xenopus laevis from the transplantation of single somatic nuclei. Nature 182, 64–65. (doi:10.1038/182064a0)
Hahn, W. C. &Weinberg, R. A. 2002 Modelling the molecular circuitry of cancer. Nat. Rev. Cancer 2, 331–341. (doi:10.1038/nrc795)
Hall, P. A. &Watt, F. M. 1989 Stem cells: the generation and maintenance of cellular diversity. Development 106, 619–633.
Hochedlinger, K., Yamada, Y., Beard, C. & Jaenisch, R. 2005 Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell 121, 465–477. (doi:10.1016/j.cell.2005.02.018)
Hyun, I. et al. 2008 New ISSCR guidelines underscore major principles for responsible translational stem cell research. Cell Stem Cell 3, 607–609. (doi:10.1016/j.stem.2008.11.009)
Ivanova, N. B., Dimos, J. T., Schaniel, C., Hackney, J. A., Moore, K. A. & Lemischka, I. R. 2002 A stem cell molecular signature. Science 298, 601–604. (doi:10. 1126/science.1073823) ccc
Izumi, N., Era, T., Akimaru, H., Yasunaga, M. & Nishikawa, S. 2007 Dissecting the molecular hierarchy for mesendoderm differentiation through a combination of embryonic stem cell culture and RNA interference. Stem Cells 25, 1664–1674. (doi:10.1634/stemcells.2006-0681)
Jaenisch, R. & Young, R. 2008 Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell 132, 567–582. (doi:10.1016/j.cell.2008.01.015)
Jensen, K. B. & Watt, F. M. 2006 Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence. Proc. Natl. Acad. Sci. USA 103, 11958–11963. (doi:10.1073/ pnas.0601886103)
Kamitakahara, M., Ohtsuki, C. & Miyazaki, T. 2008 Review paper: behavior of ceramic biomaterials derived from tricalcium phosphate in physiological condition. J. Biomater. Appl. 23, 197–212. (doi:10.1177/ 0885328208096798)
Kerr, C. L., Gearhart, J. D., Elliott, A. M. & Donovan, P. J. 2006 Embryonic germ cells: when germ cells become stem cells. Semin. Reprod. Med. 24, 304–313. (doi:10.1055/s-2006-952152)
Kim, J. B. et al. 2008 Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors. Nature 454, 646–650. (doi:10.1038/nature07061)
Lajtha, L. G. 1979 Stem cell concepts. Nouv. Rev. Fr. Hematol. 21, 59–65. Lau, D., Ogbogu, U., Taylor, B., Stafinski, T., Menon, D. & Caulfield, T. 2008 Stem cell clinics online: the direct-toconsumer portrayal of stem cell medicine. Cell Stem Cell 3, 591–594. (doi:10.1016/j.stem.2008.11.001)
Leblond, C. P. 1964 Classification of cell populations on the basis of their proliferative behavior. Natl. Cancer Inst. Monogr. 14, 119–150.
Lo Celso, C. et al. 2009 Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. Nature 457, 92–96. (doi:10.1038/nature07434)
Lowell, S., Benchoua, A., Heavey, B. & Smith, A. G. 2006 Notch promotes neural lineage entry by pluripotent embryonic stem cells. PLoS Biol. 4, e121. (doi:10.1371/journal.pbio.0040121)
Macchiarini, P. et al. 2008 Clinical transplantation of a tissue-engineered airway. Lancet 372, 2023–2030. (doi:10.1016/S0140-6736(08)61598-6)
Markoulaki, S. et al. 2009 Transgenic mice with defined combinations of drug-inducible reprogramming factors. Nat. Biotechnol. 27, 169–171. (doi:10.1038/nbt.1520)
Marson, A. et al. 2008 Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells. Cell 134, 521–533. (doi:10.1016/j.cell.2008.07.020)
Martin, G. 1981 Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. USA 78, 7634–7638. (doi:10.1073/pnas.78.12.7634)
Mathur, D., Danford, T. W., Boyer, L. A., Young, R. A., Gifford, D. K. & Jaenisch, R. 2008 Analysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET. Genome Biol. 9, R126. (doi:10.1186/gb-2008-9-8-r126)
Mavilio, F. et al. 2006 Correction of junctional epidermolysis bullosa by transplantation of genetically modified epidermal stem cells. Nat. Med. 12, 1397–1402. (doi:10.1038/nm1504)
McCulloch, E. A., Minden, M. D., Miyauchi, J., Kelleher, C. A. & Wang, C. 1988 Stem cell renewal and differentiation in acute myeloblastic leukaemia. Review. J. Cell Sci. Suppl. 10, 267–281.
Meissner, A. et al. 2008 Genome-scale DNA methylation maps of pluripotent and differentiated cells. Nature 454, 766–770.
Miller, R. A. & Ruddle, F. H. 1976 Pluripotent teratocarcinoma-thymus somatic cell hybrids. Cell 9, 45–55. (doi:10.1016/0092-8674(76)90051-9)
Morrison, S. J. & Spradling, A. C. 2008 Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. Cell 132, 598–611. (doi:10.1016/j.cell. 2008.01.038)
O’Brien, C. A., Pollett, A., Gallinger, S. & Dick, J. E. 2007 A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature 445, 106–110. (doi:10.1038/nature05372)
Okita, K., Nakagawa, M., Hyenjong, H., Ichisaka, T. & Yamanaka, S. 2008 Generation of mouse induced pluripotent stem cells without viral vectors. Science 322, 949–953. (doi:10.1126/science.1164270)
Park, I. H., Zhao, R., West, J. A., Yabuuchi, A., Huo, H., Ince, T. A., Lerou, P. H., Lensch, M. W. & Daley, G. Q. 2008a Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451, 141–146. (doi:10.1038/nature06534)
Park, I. H. et al. 2008b Disease-specific induced pluripotent stem cells. Cell 134, 877–886. (doi:10.1016/j.cell.2008.07.041)
Perry, A. R. & Linch, D. C. 1996 The history of bonemarrow transplantation. Blood Rev. 10, 215–219. (doi:10.1016/S0268-960X(96)90004-1)
Pike-Overzet, K., van der Burg, M., Wagemaker, G., van Dongen, J. J. & Staal, F. J. 2007 New insights and unresolved issues regarding insertional mutagenesis in X-linked SCID gene therapy. Mol. Ther. 15, 1910–1916. (doi:10.1038/sj.mt.6300297)
Potten, C. S. & Loeffler, M. 2008 Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. Development 110, 1001–1020.
Quintana, E., Shackleton, M., Sabel, M. S., Fullen, D. R., Johnson, T. M. & Morrison, S. J. 2008 Efficient tumour formation by single human melanoma cells. Nature 456, 593–598. (doi:10.1038/nature07567)
Ramalho-Santos, M., Yoon, S., Matsuzaki, Y., Mulligan, R. C. & Melton, D. A. 2002 ‘Stemness’: transcriptional profiling of embryonic and adult stem cells. Science 298, 597–600. (doi:10.1126/science.1072530)
Reya, T., Morrison, S. J., Clarke, M. F. & Weissman, I. L. 2001 Stem cells, cancer, and cancer stem cells. Nature 414, 105–111. (doi:10.1038/35102167)
Sacco, A., Doyonnas, R., Kraft, P., Vitorovic, S. & Blau, H. M. 2008 Self-renewal and expansion of single transplanted muscle stem cells. Nature 456, 502–506. (doi:10.1038/nature07384)
Silva, J. & Smith, A. 2008 Capturing pluripotency. Cell 132, 532–536. (doi:10.1016/j.cell.2008.02.006)
Silva, J., Barrandon, O., Nichols, J., Kawaguchi, J., Theunissen, T. W. & Smith, A. 2008 Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253. (doi:10.1371/journal. pbio.0060253)
Silva-Vargas, V., Lo Celso, C., Giangreco, A., Ofstad, T., Prowse, D. M., Braun, K. M. & Watt, F. M. 2005 b-Catenin and Hedgehog signal strength can specify number and location of hair follicles in adult epidermis without recruitment of bulge stem cells. Dev. Cell 9, 121–131. (doi:10.1016/j.devcel.2005.04.013)
Singh, S. K., Clarke, I. D., Hide, T. & Dirks, P. B. 2004 Cancer stem cells in nervous system tumors. Oncogene 23, 7267–7273. (doi:10.1038/sj.onc.1207946)
Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G. & Hochedlinger, K. 2008 Induced pluripotent stem cells generated without viral integration. Science 322, 945–949. (doi:10.1126/science.1162494)
Takahashi, K. & Yamanaka, S. 2006 Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663–676. (doi:10.1016/j.cell.2006.07.024)
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K. & Yamanaka, S. 2007 Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861–872. (doi:10.1016/j.cell.2007.11.019)
Tesar, P. J., Chenoweth, J. G., Brook, F. A., Davies, T. J., Evans, E. P., Mack, D. L., Gardner, R. L. & Mckay, R. D. 2007 New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature 448, 196–199. (doi:10.1038/nature05972)
The´ry, M., Racine, V., Pe´pin, A., Piel, M., Chen, Y., Sibarita, J. B. & Bornens, M. 2005 The extracellular matrix guides the orientation of the cell division axis. Nat. Cell Biol. 7, 947–953. (doi:10.1038/ncb1307)
Thomson, J., Itskovitz-Eldor, J., Shapiro, S., Waknitz, M., Swiergiel, J., Marshall, V. & Jones, J. 1998 Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147. (doi:10.1126/science.282.5391.1145)
Torres, J. & Watt, F. M. 2008 Nanog maintains pluripotency of mouse embryonic stem cells by inhibiting NFkappaB and cooperating with Stat3. Nat. Cell Biol. 10, 194–201. (doi:10.1038/ncb1680)
Watt, F. M. 1999 Stem cell manifesto. Book review. Cell 96, 470–473. (doi:10.1016/S0092-8674(00)80643-1)
Watt, F. M. & Collins, C. A. 2008 Role of b-catenin in epidermal stem cell expansion, lineage selection, and cancer. Cold Spring Harb. Symp. Quant. Biol. 73, 503–512. (doi:10.1101/sqb.2008.73.011)
Watt, F. M. & Hogan, B. L. 2000 Out of Eden: stem cells and their niches. Science 287, 1427–1430. (doi:10.1126/science.287.5457.1427)
Watt, F. M., Jordan, P. W. & O’Neill, C. H. 1988 Cellshape controls terminal differentiation of human epidermal keratinocytes. Proc. Natl. Acad. Sci. USA 85, 5576–5580. (doi:10.1073/pnas.85.15.5576)
Wernig, M., Meissner, A., Foreman, R., Brambrink, T., Ku, M., Hochedlinger, K., Bernstein, B. E. & Jaenisch, R. 2007 In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448, 318–324. (doi:10. 1038/nature05944)
Wilmut, I., Schnieke, A. E., McWhir, J., Kind, A. J. & Campbell, K. H. 1997 Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810–813. [Erratum in Nature 1997 386, 200.] (doi:10.1038/385810a0).
Wright, B. L. & Barker, R. A. 2007 Established and emerging therapies for Huntington’s disease. Curr. Mol. Med.7, 579–587. (doi:10.2174/156652407781695738)
Xie, Y. et al. 2009 Detection of functional haematopoietic stem cell niche using real-time imaging. Nature 457, 97–101. (doi:10.1038/nature07639)
Yamanaka, S. 2007 Strategies and new developments in the generation of patient-specific pluripotent stem cells. Cell Stem Cell 1, 39–49. (doi:10.1016/j.stem.2007.05.012)
Yu, J. et al. 2007 Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917–1920. (doi:10.1126/science.1151526)
Zaret, K. S. & Grompe, M. 2008 Generation and regeneration of cells of the liver and pancreas. Science 322, 1490–1494. (doi:10.1126/science.1161431)
Zhao, C., Deng, W. & Gage, F. H. 2008 Mechanisms and functional implications of adult neurogenesis. Cell 132, 645–660. (doi:10.1016/j.cell.2008.01.033)
Zhou, Q., Brown, J., Kanarek, A., Rajagopal, J. & Melton, D. A. 2008 In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Nature 455, 627–632. (doi:10.1038/nature07314)
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دوره 3، شماره 5 - شماره پیاپی 5
خرداد 1398صفحه 44-55
- تاریخ دریافت: 06 بهمن 1397
- تاریخ بازنگری: 06 اردیبهشت 1399
- تاریخ پذیرش: 06 اردیبهشت 1399